Composite

Part:BBa_I716020:Design

Designed by: Arthur Yu   Group: iGEM07_Berkeley_UC   (2007-07-24)


(pBca1176) (RFPcass-CmR-colE1) on I716101


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1813
    Illegal PstI site found at 1827
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1813
    Illegal PstI site found at 1827
    Illegal NotI site found at 1820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal BamHI site found at 911
    Illegal XhoI site found at 2693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1813
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1813
    Illegal PstI site found at 1827
    Illegal AgeI site found at 649
    Illegal AgeI site found at 761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4876


Design Notes

PCR ca1176F/R on pSB1A2-Bca1016     (1798 bp, BamHI/XhoI/DpnI) 
Sub into I716101                    (BamHI/XhoI, 4051+9, L)
Product is pBca1176 (Select on Cam/Amp)
---------------------------------
ca1176F    Forward BamHI for CmR/colE1    gctctGGATCCggaataggaacttcatttaaatg 
ca1176R    Reverse XhoI for CmR/ColE1    cgtaaCTCGAGaagatcctttttgataatc


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

See design

References